Journal: Life

Article Title: Salivary Microbiota Is Significantly Less Diverse in Patients with Chronic Spontaneous Urticaria Compared to Healthy Controls: Preliminary Results

doi: 10.3390/life11121329

Figure Lengend Snippet: Boxplot was used to display the differences in alpha diversity visually. The Shannon index was significantly lower in Chronic Spontaneous Urticaria (CSU) group compared to the HC group. ( a ) HhaI -digestion. ( b ) MspI -digestion.

Article Snippet: 120 ng of purified PCR product was digested separately by HhaI and MspI enzymes (Thermo Fisher Scientific, Waltham, MA, USA) [ ].

Techniques:

Journal: Life

Article Title: Salivary Microbiota Is Significantly Less Diverse in Patients with Chronic Spontaneous Urticaria Compared to Healthy Controls: Preliminary Results

doi: 10.3390/life11121329

Figure Lengend Snippet: Dendogram of the salivary microbiota profiles of Chronic Spontaneous Urticaria (CSU) patients and HC. ( a ) HhaI -digestion. ( b ) MspI -digestion.

Article Snippet: 120 ng of purified PCR product was digested separately by HhaI and MspI enzymes (Thermo Fisher Scientific, Waltham, MA, USA) [ ].

Techniques:

Journal: Marine Drugs

Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins

doi: 10.3390/md14110207

Figure Lengend Snippet: Effect of various DSP toxins on protein phosphatase activity. The activities of protein phosphatases ( A ) PP2a, ( B ) PP1, and ( C ) PP5 were assessed in the presence of various concentrations of natural OA, natural DTX1, natural DTX2, synthetic DTX2, or synthetic 2- epi -DTX2. All data (mean ± SE; n = 3 or 4) were normalized to the control. Three-parameter, variable slope, non-linear dose-response analysis was performed and IC 50 and 95% confidence interval values were calculated . Note: Some of these data have been published previously in Pang et al. .

Article Snippet: Briefly, the assay tests the ability of toxins to inhibit the activity of purified PP2A (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-111; human red blood cells), purified PP1 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-110; rabbit skeletal muscle), or purified PP5 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-778; recombinant enzyme expressed in E. coli cells) against the fluorimetric substrate, 6,8-difluro-4-methyl umbelliferyl phosphate (DiFMUP; 50 μM final) (Invitrogen ThermoFisher Scientific, Waltham, MA, USA, cat. #D6567).

Techniques: Activity Assay, Control

Journal: Marine Drugs

Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins

doi: 10.3390/md14110207

Figure Lengend Snippet: Calculated IC 50 values and relative potencies for natural OA, DTX1, and DTX2, and synthetic DTX2, and 2 -epi -DTX2, based on PP2a, PP1, and PP5 inhibition.

Article Snippet: Briefly, the assay tests the ability of toxins to inhibit the activity of purified PP2A (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-111; human red blood cells), purified PP1 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-110; rabbit skeletal muscle), or purified PP5 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-778; recombinant enzyme expressed in E. coli cells) against the fluorimetric substrate, 6,8-difluro-4-methyl umbelliferyl phosphate (DiFMUP; 50 μM final) (Invitrogen ThermoFisher Scientific, Waltham, MA, USA, cat. #D6567).

Techniques: Inhibition